(1)   MEDICAL CYBERNETIC ELEMENTS IN COMPUTERIZED ASSISTED INTENSIVE THERAPY IN DOGS

(2)   VISUAL PARADIGM OVER LOGICAL CIRCUITS IN VETERINARY MEDICINE

(3)   OBSERVATIONS FOR OBTEINING THE ULTRAFINS SECTIONS ON THE LKB ULTROTOME

(4)   RESULTS OF DIGITALIZED PRICESSING ON ELECTRONMICROSCOPICALY IMAGES USING THE DEDICATED PROGRAM IMAGE PRO PLUS (VER4.5) AND THE GRAPHIC EDITOR ADOBE PHOTOSHOP (VER7.0B)

 

 

 

MEDICAL CYBERNETIC ELEMENTS

IN COMPUTERIZED ASSISTED INTENSIVE THERAPY IN DOGS

 

Authors: Cumpănaşu Florin, Surdu Cristina, Marcoci Mihaela Steliana, Preoteasa Luciana

Scientific coordinators: Lecturer, Giurgiu Gavril

University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Faculty of Veterinary Medicine, 3-5 Manastur Street, 400372, Cluj-Napoca, Romania, email: start_florin@yahoo.com

 

SUMMARY

 

The performances of a computerized assisted intensive therapy relay on the logical consistency of the cybernetics circuit used by the involved artificial intelligence agent involved.

To explore the possibility to transpose the real homeostatic cybernetics into an informational environment, we developed a medical Integrated Development Environment (IDE) composed of a relational database and the needed tools used for virtualization, testing and simulation.

Our IDE is based on the uses of an intermediate language between the natural language and the numerical internal representation of the artificial intelligence agent. From the point of view of a human agent, the direct usage of the IDE is limited by the intermediate language grammatical requirements. From the point of view of a developer, the intermediate restricted language is useful for 1) message forwarding; 2) decision priority transfer as control-flow; 3) threading complex tasks; 4) manipulating artificial effectors; 5) system integration with tried party application; 6) test and log events;

The capability of the computerized agent to virtualizes and handle relations between fundamental physiological processes is exemplified in the case of query derived usage of the IDE. We considered essential for the dog intensive therapy, some logical circuits of medical cybernetics related to the post-aggressive systemic reaction. In respect with this assumption we structured the informational-decisional system that explores cybernetic relations related to hypoxia, metabolic acidosis, tachycardia, arterial hypotension, adrenaline, glycemia and calcemia.

Further development guidelines for this theme involve the basic arithmetic computation compatibility, basic logical operations compatibility and the development of a programming language for medical use that can be used based on medical knowledge.

 

References:

1. James K.; James S. (1998). Mathematical Physilogy. Springer-Verlag, New-York.

2. Teodorescu E. (1968). Agresologie Chirurgicală. Editura Medicală, Bucureşti.

3. Diculescu I., D. Onicescu (1987). Histologie medical. Ed. Medicală Bucureşti.

 

 

(1)   MEDICAL CYBERNETIC ELEMENTS IN COMPUTERIZED ASSISTED INTENSIVE THERAPY IN DOGS

(2)   VISUAL PARADIGM OVER LOGICAL CIRCUITS IN VETERINARY MEDICINE

(3)   OBSERVATIONS FOR OBTEINING THE ULTRAFINS SECTIONS ON THE LKB ULTROTOME

(4)   RESULTS OF DIGITALIZED PRICESSING ON ELECTRONMICROSCOPICALY IMAGES USING THE DEDICATED PROGRAM IMAGE PRO PLUS (VER4.5) AND THE GRAPHIC EDITOR ADOBE PHOTOSHOP (VER7.0B)

 

 


VISUAL PARADIGM OVER LOGICAL CIRCUITS

IN VETERINARY MEDICINE

 

Authors: Drăgan Gabriela, Aoanei Monica Adriana, Cumpănaşu Florin

Scientific coordinators: Lecturer, Giurgiu Gavril

University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Faculty of Veterinary Medicine, 3-5 Manastur Street, 400372, Cluj-Napoca, Romania, email: gabi_18d@yahoo.com

 

SUMMARY

 

Decisions in veterinary practice are based on a good manage of logical circuits. Short time of reaction can be obtained if the overall image that encloses the physiology, physiopathology and metabolism can be recalled. The subject of this work is to explore the basic logical circuits of animal homeostasis trough a visual paradigm over the logical circuits involved.

To represent dynamic processes in a static context, unified modeling language UML is used from inside Visual Paradigm trail. Use case diagrams, class diagrams, object diagrams and state machine diagrams are generated. To represent dynamic processes in a dynamic content Wolfram Mathematica trail and 3DS max Studio from Autodesk trail are used.

Our main concern is directed toward the representation of hypoxia, metabolic acidosis, tachycardia, arterial hypotension, adrenaline, glycemia and calcemia and their interdependences. A simple case is that of the enclosed circle created by collagen hydratation acidic environment, increased edema, decreased tissue perfusion, increased hypoxia, increased metabolic acidity.

Because of the UML used to create diagrams, we maintain the compatibility with other unified modeling language based environments. Class and object diagrams are used in our case to describe the normal composition of the body environment and the elements involved in physiological processes. Machine stare diagram are used to decompose and represent complex pathologic events and to show the spatiality of processes. UML implementations allow us to aggregate blocks of structures for better interpretation.

Further development guidelines for this theme involve a wider area of phenomena represented in the visual paradigm as well as the development of tools than can work with existent data to output interactive diagrams.

Because the unrestricted natural medical language, there are many indeterminate or partially determined relations between elements that make the object of natural medical language.

References

1. James K.; James S. (1998). Mathematical Physilogy. Springer-Verlag, New-York.

2. Teodorescu E. (1968). Agresologie Chirurgicală. Editura Medicală, Bucureşti.

3. Diculescu I., D. Onicescu (1987). Histologie medical. Ed. Medicală Bucureşti.

 

(1)   MEDICAL CYBERNETIC ELEMENTS IN COMPUTERIZED ASSISTED INTENSIVE THERAPY IN DOGS

(2)   VISUAL PARADIGM OVER LOGICAL CIRCUITS IN VETERINARY MEDICINE

(3)   OBSERVATIONS FOR OBTEINING THE ULTRAFINS SECTIONS ON THE LKB ULTROTOME

(4)   RESULTS OF DIGITALIZED PRICESSING ON ELECTRONMICROSCOPICALY IMAGES USING THE DEDICATED PROGRAM IMAGE PRO PLUS (VER4.5) AND THE GRAPHIC EDITOR ADOBE PHOTOSHOP (VER7.0B)

 

 


Buletinul USAMV-CN,

ISSN 1454-2382

 

Cumpănaşu F. ; V. Rus 

 

Universitatea de Ştiinţe Agricole şi Medicină Veterinară

Facultatea de Medicină Veterinară

Cluj-Napoca

 

 

Observations for obteining the ultrafins sections on the LKB ultrotome

 

SUMMARY

 

During 11-03-2004/04.05.2004 in a stage of professional training, I have been attending a course of electronic microscopy at the Center Of Electronic Microscopy at Babes-Bolyai University. I have effectively participated at the making of glass knives, at the modeling of blocks for sectioning, at obtaining the ultra-fine sections using LKB ultra-microtome and at contrasting the sections to examine at transmission electronic microscope.

During this manual labors I have confronted various technical problems, and I have solved these after many hours of exercises and attentive observation. The solution may be: Diving of the sections is remedying through a lowering water lever from the lake. The appearance of the ununiform sections in thickness is remedying trough the remodeling of the specimen closer the piece margins there where the fixer and the resin actioned uniformly. The obtaining of the sections with incomplete surface is prevented through modeling of resin blocks with a day before the sectioning, so that the resin ends its polymerization. The dispersion of the sections on the water surfaces is remedying through degreasing of all tools.

 Observations: The obtaining of the ultra-fine sections at LKB ultra microtome is a delicate operation that needs a special attention for each step of sectioning and a diferent way of making for each specimen.

 

Observaţii privind obţinerea secţiunilor ultrafine la microtomul LKB

 

REZUMAT

 

În perioada 11-03-2004/04.05.2004 în cadrul unui program de pregătire profesională am urmat un curs de tehnică electronomicropică în cadrul Centrului de Microscopie Electoronică al Universităţii Babeş-Bolyai. Am participat efectiv la confecţionarea cuţitelor de sticlă, fasonarea blocurilor în vederea secţionării, la obţinerea secţiunilor ultrafine.

În timpul acestor manopere m-am confruntat cu variate probleme tehnice, pentru care, după ore de exerciţii şi observaţie atentă am găsit soluţii de remediere: Alunecarea secţiunilor sub apă se remediază prin coborârea nivelului apei din bacul de colectare. Apariţia secţiunilor neuniforme ca grosime se remediază prin remodelarea specimenului, mai aproape de periferia piesei. Obţinerea secţiunilor cu suprafaţă incompletă se preîntâmpină prin fasonarea blocurilor de răşină cu o zi înainta secţionării pentru ca răşina îşi încheie polimerizarea. Dispersia secţiunilor pe suprafaţa apei se remediază prin degresarea instrumentarului de laborator.

            Concluzii: Obţinerea secţiunilor ultrafine la ultramicrotomul LKB este o operaţiune delicată care necesită acordare unei atenţii deosebite fiecărei etape a secţionării şi acomodarea modului de lucru la cerinţele fiecărui preparat.

 

 

(1)   MEDICAL CYBERNETIC ELEMENTS IN COMPUTERIZED ASSISTED INTENSIVE THERAPY IN DOGS

(2)   VISUAL PARADIGM OVER LOGICAL CIRCUITS IN VETERINARY MEDICINE

(3)   OBSERVATIONS FOR OBTEINING THE ULTRAFINS SECTIONS ON THE LKB ULTROTOME

(4)   RESULTS OF DIGITALIZED PRICESSING ON ELECTRONMICROSCOPICALY IMAGES USING THE DEDICATED PROGRAM IMAGE PRO PLUS (VER4.5) AND THE GRAPHIC EDITOR ADOBE PHOTOSHOP (VER7.0B)

 


 

Buletinul USAMV-CN,

ISSN 1454-2382

 

Cumpănaşu F. ; V. Rus 

 

Universitatea de Ştiinţe Agricole şi Medicină Veterinară

Facultatea de Medicină Veterinară

Cluj-Napoca

 

 

Results of digitalized pricessing on electronmicroscopicaly images using the dedicated program Image Pro Plus (ver4.5) and the graphic editor Adobe Photoshop (ver7.0b)

 

SUMMARY

 

The image from the  electron microscop is rich in information for morphologists. For the others, the information conntent is often unaccesible. Even interdisciplinary informational exchange need  a complementary representation. Based on aditional kit of information, achieved by the mean of objectiv methodes, every researcher can help for image interpretation, whatever his domain of activity is. The results mediatization success is emphasize by the simple nature of  aditional information.

The results are obtained trough digital image analizing on Electron Microscopy  Center of Babes-Bolyai University: Trough 3D representation I estimate the local density  of the Bowman membrane. Measuring the light intensity I create the graphic profile of a  section trough a collagen fiber. Editing the  color  palette color table I obtain indexed color image of  a liphosom. Using convolution filters I selectiv emphasize hepatocite components.

Observation: Digital image analysis is an impurtant step for research used for interdisciplinary reports, for menteining and mediatization of investigation results.

 

 

Rezultate ale prelucrării digitalizate a imaginilor electronomicroscopice cu ajutorul programului dedicat Image Pro Plus (ver4.5) şi a editorului grafic Adobe Photoshop (ver7.0b)

 

REZUMAT

 

Imaginea preluata le la microscopul electronic este bogataă în informaţie accesibilă morfologilor. Pentru toţi ceilalţi, informaţia conţinută este adesea inaccesibila. Chiar schimbul interdisciplinar de informaţie are nevoie de o completare a imaginii originale electronomicroscopice. Ajutat fiind de un set de informaţie adiţional, obţinut prin metode obiective, orice cercetator îşi poate aduce contribuţia la interpretarea imaginii electronomicroscopice în funcţie de priceperea sa dar indiferent de domeniul de activitate. Succesul mediatizarii rezultatelor studiilor electronomicroscopiice este sensibil amplificat de natura simplificată a ifrormaţiei adiţionale.

            Rezultate obţinute prin analiza digitalizată de imagini din arhiva Centrului de Microscopie Electronică al Universităţii Babeş-Bolyai: Prin reprezentări tridimensionale (shaded surface) am evaluat denditatea locală a capsulei Bowman. Măsurând intensitatea luminoasă (pathline) am determinat profilul grafic al unei secţiuni prin molecula de colagen. Editând tabela de culori (LUT) am obţinut reprezentări color ale aspectului electronomicroscopic al liposomilor. Folosind metode rgafice de filtrare (convolution type) am evidenţiat structuri morfologice ale hepatociteului.

Definind clase de obiecte am separat şi grupat statistic ribozomi şi mitocondrii pe o secţiune prin hepatocit.

            Observaţie: Analiza digitalizată a imaginilor reprezintă o etapă importantă a cercetării

utilă protocoalelor de comunicare, susţinere şi mediatizare a rezultatelor investigaţiilor electronomicroscopice.

 

(1)   MEDICAL CYBERNETIC ELEMENTS IN COMPUTERIZED ASSISTED INTENSIVE THERAPY IN DOGS

(2)   VISUAL PARADIGM OVER LOGICAL CIRCUITS IN VETERINARY MEDICINE

(3)   OBSERVATIONS FOR OBTEINING THE ULTRAFINS SECTIONS ON THE LKB ULTROTOME

(4)   RESULTS OF DIGITALIZED PRICESSING ON ELECTRONMICROSCOPICALY IMAGES USING THE DEDICATED PROGRAM IMAGE PRO PLUS (VER4.5) AND THE GRAPHIC EDITOR ADOBE PHOTOSHOP (VER7.0B)